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Circadian release of pre-stored histamine (a) and de novo synthesized cysteinyl leukotrienes (cysLT) (b) by human intestinal mast cells (hiMC) in response to FcεRI cross-linking. The hiMCs were synchronized with 40 μm dexamethasone for 2 hr and stimulated by FcεRI cross-linking every 4 hr around the circadian cycle. The release of histamine is shown in per cent of the total cell content for every time-point, n = 2, release of cysLT, n = 4, hiMC purity was > 99%.
