Figure 3.

Different profiles of cellular immune responses against interphotoreceptor retinoid-binding protein (IRBP) of DNAX-activation protein 12 (DAP12) -deficient (Tyrobp^−/−) mice and their wild-type (WT) controls from the two animal facilities. (a) Draining lymph node lymphocytes collected 14 days post-immunization were cultured with IRBP (10 μg/ml) for 48 hr and their supernatants were analysed for the indicated cytokines by Multiplex SearchLight technology. The data, in pg/ml, are means ± SEM of three experiments with mice of the first facility and two experiments with mice of the second facilty. (b) Cytokine levels (pg/ml) measured by ELISA. The bars present means ± SEM of four independent experiments. (c) Opposite profiles of proliferative responses against IRBP in cultures of DAP12-deficient mice and their WT controls from the two facilities. The draining lymph node lymphocytes were stimulated with IRBP at the indicated concentrations and their response measured by [³H]thymidine incorporation. The data shown are of representative experiments; similar patterns of response were observed in two additional experiments with cells of mice from each of the two facilities.