Table 1.
Studies examining PDE5 expression in the bladder
| Publication year/First author |
Species | Detection method |
Expression level |
Expression location |
Source of PDE5 Antibody |
Tension reversion |
Additional notes |
|---|---|---|---|---|---|---|---|
| 1996/Truss[15] | Human | Organ bath | ND | ND | NA | Minor effect by zaprinast | |
| 1997/Longhurst[16] | Guinea pig | Organ bath | ND | ND | NA | Zaprinast suppressed carbachol effect | Zaprinast did not suppress ATP or KCL effect |
| 1998/Stacey[32] | Human | Northern | ND | ND | NA | ND | |
| 2000/Lin[33] | Human | PCR | ND | ND | NA | ND | |
| 2002/Wibberley[17] | rat | Organ bath | ND | ND | NA | Zaprinast had no effect | |
| 2003/Lin[34] | Rat | PCR & Northern | PDE5A2 > PDE5A1 | ND | NA | ND | |
| 2004/Gillespie[18] | Guinea pig | Organ bath | ND | ND | NA | Zaprinast had no effect | Dipyridamole had transient effect |
| 2004/Morelli[35] | Human | qPCR & Western | Among the highest | ND | Dr. Giorgi | ND | Same level as CC |
| 2006/Tinel[19] | Rat | qPCR & Organ bath | Among the highest | ND | NA | Vardenafil > Sildenafil > Tadalafil | |
| 2007/Filippi[20] | Human & Rat | qPCR, IHC & Organ bath | Among the highest | Detrusor & vascular smooth muscle & endothelium | Dr. Giorgi | Vardenafil (100nM) | |
| 2008/Yanai[21] | Guinea pig | Organ bath | ND | ND | NA | Sildenafil had partial effect | |
| 2009/Werkstrom[22] | Rat | Organ bath | ND | ND | NA | Vardenafil (100µM) | |
| 2010/Lee[23] | Rabbit | Organ bath | ND | ND | NA | Udenafil (10mM) | |
| 2010/Lin[37] | Rat | IF | High | Detrusor smooth muscle | Dr. Visweswariah | ND | |
| 2011/Muller[36] | Rat | Western | Among the highest | ND | Cell Signaling Technology | ND | |
| 2012/Angula[24] | Human | Organ bath | ND | ND | NA | Tadalafil (30–60nM) potentiated SNP-induced relaxation |
CC: Corpus cavernosum; PCR: Reverse transcription-polymerase chain reaction; qPCR: Quantitative PCR; IF: Immunofluorescence; IHC: Immunohistochemistry; SNP: Sodium nitroprusside; ND: Not determined; NA: Not applicable.