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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Urology. 2013 Jan 17;81(3):480–487. doi: 10.1016/j.urology.2012.11.028

Table 2.

Studies examining PDE5 expression in the prostate

Publication
year/First author
Species Detection
method
Expression
level
Expression
location
Source of
PDE5
Antibody
Tension
reversion
Additional notes
1998/Stacey[32] Human Northern ND ND NA ND
1999/Kotera[45] Human PCR PDE5A2 > PDE5A1 ND NA ND
2000/Lin[33] Human PCR ND ND NA ND
2001/Uckert[38] Human PCR, cGMP hydrolysis & Organ bath ND ND NA Zaprinast > Sildenafil (0.1–100µM) Both drugs reversed <30% tension at 100µM
2003/Lin[34] Rat PCR & Northern PDE5A2 > PDE5A1 ND NA ND
2004/Morelli[35] Human qPCR & Western Among the highest ND Dr. Giorgi ND Same level as CC
2005/Zhang[46] Rat qPCR & Western Medium ND Dr. Giorgi ND Half level as CC
2006/Uckert[48] Human IF ND Glandular & subglandular Dr. Omori ND
2006/Tinel[19] Rat qPCR & Organ bath Among the lowest ND NA Vardenafil > Sildenafil > Tadalafil
2010/Fibbi[42] Human qPCR & IHC Medium Vascular smooth muscle & endothelium Dr. Giorgi ND
2010/Zenzmaier[49] Human IHC ND Stroma; not epithelium Cell Signaling Technology ND PDE5 peptide blocked staining
2011/Morelli[47] Human qPCR & IHC Low Vascular smooth muscle & endothelium Dr. Giorgi ND One-quarter level as CC
2011/Muller[36] Rat Western Extremely low ND Cell Signaling Technology ND
2012/Zhang[43] Rat qPCR, Western, IHC, & Organ bath Abundant by PCR & Western; scanty by IHC Fibromuscular stroma Transduction Laboratories Zaprinast at 20 nM potentiated SNP effect

CC: Corpus cavernosum; PCR: Reverse transcription-polymerase chain reaction; qPCR: Quantitative PCR; IF: Immunofluorescence; IHC: Immunohistochemistry; SNP: Sodium nitroprusside; ND: Not determined; NA: Not applicable.