Table 3.
Studies examining PDE5 expression in the urethra
| Publication year/First author |
Species | Detection method |
Expression level |
Expression location |
Source of PDE5 Antibody |
Tension reversion |
Additional notes |
|---|---|---|---|---|---|---|---|
| 2000/Lin[33] | Human | PCR | ND | ND | NA | ND | |
| 2001/Costa[41] | Sheep | Organ bath | ND | ND | NA | Zaprinast potentiated nitrergic effect | |
| 2002/Wibberley[17] | Rat | Organ bath | ND | ND | NA | Zaprinast potentiated nitrergic effect | Involved striated muscle |
| 2003/Lin[34] | Rat | PCR | PDE5A2 > PDE5A1 | ND | NA | ND | |
| 2006/Tinel[19] | Rat | qPCR & Organ bath | Medium | ND | NA | Vardenafil > Sildenafil > Tadalafil | |
| 2006/Werkstrom[53] | Pig | IF & Organ bath | ND | Urethral & vascular smooth muscle & endothelium | FabGennix | Vardenafil & tadalafil (30µM) | |
| 2010/Lee[23] | Rabbit | Organ bath | ND | ND | NA | Udenafil (10mM) | |
| 2010/Lin[37] | Rat | IF & Organ bath | High in striated muscle | Striated > smooth muscle | Dr. Visweswariah | Sildenafil potentiated SNP effect | Levator ani also positive |
| 2010/Fibbi[42] | Human | qPCR & IHC | Medium | Smooth muscle | Dr. Giorgi | ND | |
| 2011/Morelli[47] | Human | qPCR | Medium | ND | NA | ND |
PCR: Reverse transcription-polymerase chain reaction; qPCR: Quantitative PCR; IF: Immunofluorescence; IHC: Immunohistochemistry; SNP: Sodium nitroprusside; ND: Not determined; NA: Not applicable.