Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Biomaterials. 2013 Aug 21;34(35):10.1016/j.biomaterials.2013.08.008. doi: 10.1016/j.biomaterials.2013.08.008

Figure 4.

Figure 4

Encapsulated PCs (encapsulated with an initial concentration of 16 million PCs/ml alginate) remain viable for up to 14 days in vitro, as evaluated with calcein (green), ethidium homodimer (red) and Hoechst (blue). Confocal projections are shown in A (calcein, Hoechst) and B–E (calcein, ethidium homodimer and Hoechst). (A) Day 1; (B) Day 3; (C) Day 7; (D) Day 10; (E) Day 14. Quantification of viable cell number per particle is shown in (F). Encapsulated PCs (initial concentration of 8 million PCs/ml alginate) were treated with 0.1% saponin after dying with calcein and Hoechst to provide a control (G, H). (G) Day 1; (H) Day 1, treated with 0.1% saponin, showing alginate does not retain calcein after cell death. Scale bars = 200 μm.