Fig. 5.
RNAi of frontalin biosynthesis. (A) Transcript levels for DponHMG-R, DponGPPS/FPPS, and DponGGPPS in juvenile hormone-treated male adults injected with dsRNA for DponHMG-R, DponGPPS/FPPS, and DponGGPPS. Asterisks indicate treatments that were significantly different from the control (P < 0.05, by two-tailed t test with Holm–Bonferroni correction on the ∆∆CT values, after significant one-way ANOVA tests; n = 10). (B) Frontalin levels in juvenile hormone-treated male adults injected with dsRNA for DponHMG-R, DponGPPS/FPPS, and DponGGPPS (one-way ANOVA: F3,44 = 12.1383, P < 0.00001, n = 12). Treatments with the same letter are not significantly different (P < 0.05, by two-tailed t test with Holm–Bonferroni correction).