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ATP is not required for VSD–PGD coupling. (A) VCF recordings of WT KCNQ1 (black), W379S (red), and R380S/R397W (green) with and without L353K. (B) Steady-state fluorescence change vs. voltage (F–V). Solid curves are fits of a Boltzmann equation with V1/2. WT (black solid): −53.5 ± 1.1 mV; W379S (red solid): −56.2 ± 0.8 mV; R380S/R397W (green solid): −50.3 ± 0.9 mV; L353K (black open): −72.3 ± 0.8 mV; W379S/L353K (red open): −76.9 ± 0.9 mV; R380S/R397W/L353K (green open): −70.9 ± 1.1 mV.
