Table 1.

The TIF⁻ phenotype is recessive and not caused by upsets in msl-based dosage compensation

Cross generating females*	Relevant genotype of females with P{U2af-traF}/+ (mated unless otherwise specified)	Relative viability, %†	Egg-laying parameters‡
			% laying females§	No. tested	Eggs laid⋅laying female−1⋅d−1 ± SEM¶	Maximum no. eggs laid in 1 d||
A	Sxlf7,M1/ SxlM1,fΔ33	73	0	39	–	–
A	Sxlf7,M1/ SxlM1,fΔ33; Dp(Sxl+)	ref (n = 77)	100	23	70.4 ± 3.4	59–142
A	Dp(Sxl+) but not mated		90	30	6.8 ± 1.3	3–57
B	Sxlf7,M1/ SxlM1,fΔ33; msl-2	52	4	78	0.3 ± 0.1	1–3
B	+/Sxlf7,M1; msl-2	ref (n = 272)	100	34	60.6 ± 3.8	42–120
C	P{SxlΔPm} Sxlf18,f32/Sxl f18,f32	93	89	37	7.3 ± 1.5	1–56
C	P{SxlΔPm} Sxlf18,f32/Sxl f18,f32; Dp(Sxl+)	ref (n = 207)	100	10	73.7 ± 5.2	68–126
C	Dp(Sxl+) but not mated		100	11	10.7 ± 2.0	1–74

^*Full genotype of crosses: A. y w Sxl^M1,fΔ33 ct⁶ sn³/Binsinscy; P{U2af-tra^F w^+mW.hs}2B/+ ☿☿ × ♂♂ w cm Sxl^f7,M1 ct⁶ v/Y; Dp(1;3)sn^13a1, cm⁺Sxl⁺ct⁺/+; B. y w Sxl^M1,fΔ33/Binsinscy; msl-2¹ P{U2af-tra^F w^+mW.hs}2B/msl-2¹☿☿ × ♂♂ w cm Sxl^f7,M1ct⁶ v/Y; msl-2¹/CyO; and C. y w cm P{w^+mC SxlΔPm} Sxl^f18, ^f32ct⁶/Binsinscy; P{U2af-tra^F w^+mW.hs}2B/+ ☿☿ × ♂♂ y w Sxl^f18, ^f32 ct⁶/Y; Dp(1;3)sn^13a1, cm⁺Sxl⁺ct⁺/+.

^†% viability relative to the reference class (ref) indicated (no. individuals in that reference class), based on adult eclosion. U2af-tra^F had no effect on Sxl^f7,M1/ Sxl^M1,fΔ33 viability. Only Sxl-mutant females that survived the entire 5-d laying test were included in the egg-laying calculations.

^‡Virgin females were mated with four or five virgin Ore-R males 1–2 d after eclosion and then were allowed to lay for five successive days, with transfers to fresh food each day.

^§At least one egg laid over the 5-d test period qualified a female as “laying.”

^¶Calculation based on pooled data for all 5 d of egg collection.

^||For individual laying females.