Table 2.
GAL4/UAS-driven Sxlalt5-C8 expression that rescues the TIF− mutant ovulation phenotype reveals a neuronal basis for TIF
| P{UAS-Sxlalt5-C8} and GAL4 indicated driver* |
SxlM1,fΔ33/Sxlf7,M1;P{U2af-traF}/+ females† |
SxlM1,fΔ33/Y male brothers‡ |
||
| % females laying (no. tested) | No. eggs/d ± SEM for laying females | Relative viability vs. control siblings, % | % fertile (no. tested) | |
| αTub84B | 91 (43) | 43.5 ± 2.4 | 0 | — |
| Control§ | 0 (9) | – | (n = 44) | n.d. |
| elav | 89 (27) | 28.6 ± 3.4 | 27 | 0 (13) |
| Control§ | 14 (21) | 6.0 ± 3.8 | (n = 33) | 92 (26) |
| fruP1 | 82 (17) | 17.0 ± 4.7 | 92 | 0 (12) |
| Control§ | 0 (19) | – | (n = 49) | 100 (7) |
| OK233 | 95 (20) | 29.8 ± 0.6 | 71 | 56 (17) |
| Control§ | 20% (5) | 1.5 ± 1.3 | (n = 43) | 71 (9) |
*
Cross to make tested animals: y w SxlM1,fΔ33/Binsinscy, y w sn B; P{U2af-traF w+mW.hs}2B/+; P{UAS- Sxlalt5-C8, w+mC}A1/TM3,Sb Ser ♀♀ × ♂♂ w cm Sxlf7,M1 ct v/Y; GAL4/Ki for all but OK233; y w SxlM1,fΔ33/Binsinscy, y w sn B; P{U2af-traF w+mW.hs}2B/+; P{UAS- Sxlalt5-C8, w+mC}A1/TM3,Sb Ser ♀♀ × ♂♂ w cm Sxlf7,M1 ct v/Y; Gal4/CyO or Sco for OK233.
†
Virgin females were individually mated with four or five virgin Ore-R males 1–2 d after eclosion and then were allowed to lay for 2 d.
‡
Single virgin males were mated with four or five Ore-R virgin females who were given 5 d to lay.
§
No Gal4 driver.