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. 2013 Nov 4;110(47):18982–18987. doi: 10.1073/pnas.1301262110

Fig. 4.

Fig. 4.

Glycolytic genes are specifically expressed in developing muscles and required for proper muscle development. (A) Diagram representing the 10 steps of glycolysis. Genes in bold were functionally analyzed in this study. (B) Developmental profile of PyK activity during Drosophila embryogenesis. Error bars show SD. Asterisks indicate significance of changes between stages: *0.01 < P value< 0.05. (C–I) Expression profiles of glycolytic (C–H) and Impl3 (I) genes. Lateral views of three abdominal segments from stage-15 (13 h AEL) embryos double-stained for glycolytic gene transcripts (green) and for β-3 tubulin to reveal muscles (red). Note that all glycolytic genes and Impl3 show predominantly muscular expression. (J) Wild-type muscle pattern in three abdominal hemisegments as revealed by anti–β-3 tubulin staining on stage-15 (13 h AEL) embryo. (K–Q) Effects of muscle-specific RNAi knockdown of glycolytic genes and Impl3. Representative views of three abdominal hemisegments are shown. (Scale bars: 30 µm.) Arrowheads point to unfused myoblasts.