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Current records for oocytes expressing wild-type hSGLT1 and mutant W291C. The experiments were performed with membrane potential held at −50 mV (Vh) and stepped to a series of test voltages (from +50 mV to −150 mV) for 100 ms before returning to Vh. Current records for hSGLT1 (A) and for mutant W291C (B) in 0 mM, 10 mM, and 100 mM. Na+ concentration was varied by equimolar replacement of NaCl with choline chloride.
