Fig. 6.

Synergistic effect of exemestane and sulforaphane on LPS-activated iNOS activity in mouse peritoneal macrophages. (A) Peritoneal macrophages isolated from C57BL6 mice were plated in 96-well plates. Cells were exposed to exemestane, sulforaphane, or their combinations at a 5:1 molar ratio in the presence of 100 ng/mL LPS for 24 h. NO production was measured as nitrite accumulation in the medium. Control cells were treated with LPS only. Each bar represents the mean of eight replicates. SDs for all measurements were less than 10%. (B) Combination index (CI) values were plotted against the effects (fraction affected) mediated by five different drug combinations (0.75, 1.5, 3.0, 6.0, and12.0 μM total concentration shown next to each datum point), respectively. CI values from this experiment were obtained by using CompuSyn software (29).