Fig. 2.

Knock-down of dys-1 accelerates age-dependent protein aggregation in muscle cells. (A) Protein aggregation in the muscle cells was examined using transgenic animals expressing polyQ35::YFP in the body wall muscles (AM140), which shows age-dependent aggregation (32). The AM140 strain was fed with bacteria harboring either a control (empty vector) or dys-1 RNAi-feeding vector. The L4 stage was set for day 0. The arrows indicate the polyQ35::YFP aggregates. (Magnification: 10×.) (B) PolyQ35::YFP aggregates were quantified in the control (white bars) and dys-1 (black bars) RNAi-treated AM140 animals at day 3 of adulthood. P < 0.0001, χ²; n = 27. Three independent experiments produced similar results.