Fig. 3.
DPN-treated Olig2,ERβ CKO and WT EAE mice show similar peripheral cytokine response and CNS inflammation. (A) Cytokine production by MOG35–55-stimulated splenocytes was evaluated. Treatment of Olig2,ERβ CKO and WT EAE mice with DPN had no effect on measured cytokine levels. Data are representative of experiments repeated two times, n = 3 per treatment group. Dorsal column of thoracic spinal cords was imaged at ×10 and ×40 magnification from DPN-treated Olig2,ERβ CKO EAE mice (B, i) and WT EAE mice (B, ii). Both DPN-treated genotypic cohorts exhibit a similar increase in GFAP+ (B, iii), CD45+ (B, iv), and CD3+ (B, v) intensity. *P < 0.05 ANOVAs; Bonferroni’s multiple comparison posttest; n = 8 mice per treatment group.