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. 2013 Nov 4;110(47):18898–18903. doi: 10.1073/pnas.1319482110

Fig. 1.

Fig. 1.

Equilibrium melting and kinetic folding. (A) Equilibrium GdmCl unfolding (20 °C, pH 9), with the posttransition base line compared with kinetic burst phase signals. (B) Fast and slow refolding seen by fluorescence, CD, and ANS binding (1.4 µM 8-anilino-1-naphthalenesulfonic acid). (C) Burst phase amplitude versus GdmCl. (D) Atom pair distances by SAXS after 0.7 s of folding in 0.2 M GdmCl. (Inset) Shape reconstructions. (E) Multiple charge states of burst phase MBP by ESI MS after ∼50 ms of folding compared with unfolded (4 M urea) and native MBP (0.4 M urea). (F) Final folding rate versus solvent viscosity adjusted by glycerol.