Fig. 5.

Association of Pim-1 with the αHL–PLM–TEV pore at different ionic strengths. Log k₊₂ (where k₊₂ is the second-order association rate constant) was plotted against (where is the mean activity coefficient, calculated from the extended Debye–Hückel equation). At the limit [KCl] → ∞, , and k_{+2 (basal)} ∼8 × 10⁵ M^–1⋅s^–1. Measurements were performed in 15 mM Mops, pH 6.8, with the ionic strength adjusted with KCl. The ionic strength contribution from the Mops was ∼4 mM. The applied potential was –50 mV, and the filter corner frequency was 2 kHz. Error bars represent SDs (n = 5). Dotted lines indicate the 95% prediction interval for the linear regression.