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. 2013 Nov 20;41(Pt 6):1449–1458. doi: 10.1042/BST20130196

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© 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Virus particles were adsorbed on to carbon-coated copper grids for 5 min, stained with 2% uranyl acetate, and examined using a JEM-1010 transmission electron microscope (Jeol).