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. 2013 Nov 20;41(Pt 6):1449–1458. doi: 10.1042/BST20130196

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© 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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The control sample shows the leader-proximal CRISPR region amplified from the uninfected wild-type S. solfataricus P2 strain. The larger amplified products visible for loci C, D and E from day 3, after reactivation of the culture from cells stored at −80°C, contain de novo spacers. A single upper band carrying de novo spacers, indicated by an arrow, was observed for locus A.