Figure 2.

 CXCL13:CXCR5 interaction promotes LNCaP and PC3 cell invasion independent of DOCK2. LNCaP and PC3 cells were tested for their ability to invade Matrigel^TM matrix and migrate through an 8.0 μm porous membrane in the presence of CXCL13 (100 ng/ml), isotype control antibody or anti‐human CXCR5 antibody (1 μg/ml), DOCK2 or control siRNA (2 μm). Cells which invaded to the lower surfaces of the membrane were stained with crystal violet and counted using light microscopy at 40× magnification. Percentage cell invasion was calculated following manufacturer’s instructions (BD Biosciences). Error bars represent standard error of means of three independent experiments. *Significant differences (P < 0.05) relative to CXCL13‐treated cells (control).