Figure 1.

miR 488* downregulates AR expression. Representative western blots showing the expression of AR and β-actin in LNCaP (a) and C4-2B (c) cells treated with indicated amounts of miR 488* mimic or NC mimic for 48 hr. Mock transfected cells represent cells treated with Lipofectamine 2000. β-actin expression was used as a loading control. (b and d) Quantitation of AR expression in the respective lanes of Western blots shown in (a) and (c). The signal intensities of bands were measured using the SCION IMAGE analysis software. The AR expression in each lane was determined by normalizing AR band intensity to β-actin band intensity.