Figure 3.

miR 488* downregulates AR transcriptional activity. (a) qRT-PCR analysis of PSA mRNA expression in LNCaP cells transfected with miR 488* mimic (100 nM) or NC mimic (100 nM) in the presence of DHT (20 nM). Each bar represents PSA mRNA expression normalized to GAPDH mRNA expression. (b) Secreted PSA protein levels in LNCaP cells treated as in (a). Amounts of PSA in cell culture supernatants were determined using a quantitative sandwich enzyme immunoassay technique. (a and b) Cells treated with vehicle alone or DHT (bars 1 and 2) were also treated with Lipofectamine 2000 (mock transfected). Data are plotted as mean ± SE of three independent experiments. Asterisks indicate statistical significance as determined by independent samples t-test. *p < 0.01.