Figure 4.

miR 488* inhibits the growth of PCa cells. Cell viability assay in LNCaP (a), C4-2B (b) and DU 145 (c) cells transfected with either miR 488* mimic (50 nM) or NC mimic (50 nM). Mock transfected cells represent cells treated with Lipofectamine 2000. CellTiter-Glo Luminescent Cell Viability Assay (Promega) was used to determine the number of viable cells. In this assay, the amount of ATP in viable cells is quantitated using a luciferase reaction and measurement of luminescence signal. Hence, luminescence signal serves as a measure of cell viability. Data are plotted as mean ± SE of three independent experiments. Independent samples t-test was used to assess statistically significant differences. Asterisks indicate a significant difference from NC mimic transfected cells. *p < 0.05.