Table 1.
n | Setup | N | Kd (M) | ΔH (kcal mol−1) | ΔS (cal mol−1 K−1) | ΔG (kcal mol−1) | KdCaBP1 / KdCaM | Kd(apo) / Kd (Ca2+) | Δ ΔG (kcal mol−1) | |
---|---|---|---|---|---|---|---|---|---|---|
| ||||||||||
N-lobeBP** | 3 | S | 0.82 ± 0.05 | 1.10 × 10−6 ± 8.0 × 10−8 | 2.09 ± 0.40 | 34.5 ± 1.5 | −7.84 ± 0.04 | - | - | - |
Ca2+/C-lobeBP** | 4 | S | 0.90 ± 0.07 | 1.05 × 10−8 ± 1.9 × 10−9 | −2.88 ± 0.19 | 25.5 ± 0.6 | −10.52 ± 0.11 | - | - | - |
Ca2+/CaBP1** | 2 | D | # | 2.9 × 10−10 ± 7.0 × 10−11 | −4.72 ± 0.07 | 26.6 ± 0.7 | −12.38 ± 0.14 | - | - | - |
| ||||||||||
Ca2+ /N-lobeCaM* | 2 | S | 0.85 ± 0.05 | 5.76 × 10−8 ± 3.55 × 10−8 | −1.91 ± 0.14 | 26.7 ± 0.9 | −9.60 ± 0.38 | 19 | - | - |
Ca2+ /C-lobeCaM* | 2 | S | 0.98 ± 0.18 | 2.63 × 10−9 ± 7.0 × 10−11 | −6.77 ± 0.21 | 14.2 ± 0.9 | −11.31 ± 0.01 | 4.0 | - | - |
Ca2+/CaM | 2 | D | # | 8.5 × 10−13 ± 1.3 × 10−13 | −5.74 ± 0.35 | 34.5 ± 0.9 | −15.68 ± 0.08 | 340 | - | - |
| ||||||||||
N-lobeBP** | 3 | S | 0.82 ± 0.05 | 1.10 × 10−6 ± 8.0 × 10−8 | 2.09 ± 0.40 | 34.5 ± 1.5 | −7.84 ± 0.04 | - | 1 | 0 |
C-lobe CaBP1EF34 | 2 | D | # | 3.80 × 10−6 ± 1.30 × 10−6 | 3.77 ± 0.20 | 37.7 ± 1.4 | −7.08 ± 0.21 | − | 360 | −3.44 |
CaBP1EF34 | 2 | D | # | 9.1 × 10−8 ± 1.3 × 10−8 | −0.82 ± 0.15 | 29.0 ± 0.2 | −9.15 ± 0.08 | − | 310 | −3.24 |
| ||||||||||
N-lobe CaMEF12 | 2 | D | # | > 5 × 10−6 | n/a | n/a | > −6.9 | < 0.2 | > 90 | < −2.78 |
C-lobeCaMEF34 | 2 | D | # | 2.2 × 10−6 ± 1.0 × 10−8 | −3.79 ± 0.06 | 12.4 ± 0.2 | −7.34 ± 0.02 | 1.7 | 840 | −4.05 |
CaMEF1234 | 2 | D | # | 5.8 × 10−7 ± 5.0 × 10−8 | −3.36 ± 0.02 | 16.5 ± 0.2 | −8.10 ± 0.06 | 0.14 | 680,000 | −7.58 |