Table 1.

Titration calorimetry data for interactions between CaM and CaBP1 with the Ca_V1.2 IQ domain

	n	Setup	N	Kd (M)	ΔH (kcal mol−1)	ΔS (cal mol−1 K−1)	ΔG (kcal mol−1)	KdCaBP1 / KdCaM	Kd(apo) / Kd (Ca2+)	Δ ΔG (kcal mol−1)
N-lobeBP**	3	S	0.82 ± 0.05	1.10 × 10−6 ± 8.0 × 10−8	2.09 ± 0.40	34.5 ± 1.5	−7.84 ± 0.04	-	-	-
Ca2+/C-lobeBP**	4	S	0.90 ± 0.07	1.05 × 10−8 ± 1.9 × 10−9	−2.88 ± 0.19	25.5 ± 0.6	−10.52 ± 0.11	-	-	-
Ca2+/CaBP1**	2	D	#	2.9 × 10−10 ± 7.0 × 10−11	−4.72 ± 0.07	26.6 ± 0.7	−12.38 ± 0.14	-	-	-
Ca2+ /N-lobeCaM*	2	S	0.85 ± 0.05	5.76 × 10−8 ± 3.55 × 10−8	−1.91 ± 0.14	26.7 ± 0.9	−9.60 ± 0.38	19	-	-
Ca2+ /C-lobeCaM*	2	S	0.98 ± 0.18	2.63 × 10−9 ± 7.0 × 10−11	−6.77 ± 0.21	14.2 ± 0.9	−11.31 ± 0.01	4.0	-	-
Ca2+/CaM	2	D	#	8.5 × 10−13 ± 1.3 × 10−13	−5.74 ± 0.35	34.5 ± 0.9	−15.68 ± 0.08	340	-	-
N-lobeBP**	3	S	0.82 ± 0.05	1.10 × 10−6 ± 8.0 × 10−8	2.09 ± 0.40	34.5 ± 1.5	−7.84 ± 0.04	-	1	0
C-lobe CaBP1EF34	2	D	#	3.80 × 10−6 ± 1.30 × 10−6	3.77 ± 0.20	37.7 ± 1.4	−7.08 ± 0.21	−	360	−3.44
CaBP1EF34	2	D	#	9.1 × 10−8 ± 1.3 × 10−8	−0.82 ± 0.15	29.0 ± 0.2	−9.15 ± 0.08	−	310	−3.24
N-lobe CaMEF12	2	D	#	> 5 × 10−6	n/a	n/a	> −6.9	< 0.2	> 90	< −2.78
C-lobeCaMEF34	2	D	#	2.2 × 10−6 ± 1.0 × 10−8	−3.79 ± 0.06	12.4 ± 0.2	−7.34 ± 0.02	1.7	840	−4.05
CaMEF1234	2	D	#	5.8 × 10−7 ± 5.0 × 10−8	−3.36 ± 0.02	16.5 ± 0.2	−8.10 ± 0.06	0.14	680,000	−7.58

ΔΔ G = RTln (K_d(apo) / K_d (Ca²⁺), where T = 288K

“*”denotes data from ref. ³⁰

“**”denotes data from ref. ²³

“#” denotes that N was set to 1

“S” denotes a simple and “D” a displacement titration

‘n’ denotes the number of independent experiments.

“n/a” means not applicable