Table 2.
n | Setup | N | Kd (M) | ΔH (kcal mol−1) | ΔS (cal mol−1 K−1) | ΔG (kcal mol−1) | KdIQAAA / KdIQ | ΔΔ G (kcal mol−1) | |
---|---|---|---|---|---|---|---|---|---|
| |||||||||
N-lobeCaBP1 | 2 | S | n/a | > 5 × 10−6 | n/a | n/a | > −6.9 | > 4.5 | < −0.9 |
Ca2+/C-lobeCaBP1 | 2 | S | 0.93 ± 0.01 | 2.21 × 10−7 ± 1.4 × 10−8 | −1.45 ± 0.14 | 25.5 ± 0.6 | −8.65 ± 0.03 | 21 | −1.87 |
Ca2+/CaBP1 | 2 | S | 0.89 ± 0.19 | 1.09 × 10−8 ± 2.0 × 10−9 | −4.77 ± 1.04 | 19.4 ± 4.0 | −10.35 ± 0.11 | 38 | −2.03 |
| |||||||||
Ca2+/N-lobeCaM | 2 | S | n/a | > 5 × 10−6 | n/a | n/a | > −6.9 | > 90 | < −2.7 |
Ca2+ /C-lobeCaM | 2 | S | 0.90 ± 0.01 | 1.54 × 10−9 ± 2.1 × 10−10 | −6.44 ± 0.13 | 18.0 ± 0.1 | −11.45 ± 0.08 | 0.6 | 0.14 |
Ca2+/CaM | 2 | D | # | 1.7 × 10−10 ± 2.0 × 10−11 | −10.6 ± 0.4 | 7.4 ± 1.6 | −12.68 ± 0.07 | 200 | −3.00 |
ΔΔ G = RTln (KdIQAAA / KdIQ), where T = 288K
“#” indicates that N was set to 1.
“S” denotes a simple and “D” a displacement titration
‘n’ indicates the number of independent experiments.
“n/a” means not applicable.