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. Author manuscript; available in PMC: 2014 Sep 9.
Published in final edited form as: J Mol Biol. 2013 Jun 25;425(17):10.1016/j.jmb.2013.06.024. doi: 10.1016/j.jmb.2013.06.024

Table 2.

Titration calorimetry data for interactions between CaM and CaBP1 with CaV1.2 IQ domain TripleA

n Setup N Kd (M) ΔH (kcal mol−1) ΔS (cal mol−1 K−1) ΔG (kcal mol−1) KdIQAAA / KdIQ ΔΔ G (kcal mol−1)
N-lobeCaBP1 2 S n/a > 5 × 10−6 n/a n/a > −6.9 > 4.5 < −0.9
Ca2+/C-lobeCaBP1 2 S 0.93 ± 0.01 2.21 × 10−7 ± 1.4 × 10−8 −1.45 ± 0.14 25.5 ± 0.6 −8.65 ± 0.03 21 −1.87
Ca2+/CaBP1 2 S 0.89 ± 0.19 1.09 × 10−8 ± 2.0 × 10−9 −4.77 ± 1.04 19.4 ± 4.0 −10.35 ± 0.11 38 −2.03
Ca2+/N-lobeCaM 2 S n/a > 5 × 10−6 n/a n/a > −6.9 > 90 < −2.7
Ca2+ /C-lobeCaM 2 S 0.90 ± 0.01 1.54 × 10−9 ± 2.1 × 10−10 −6.44 ± 0.13 18.0 ± 0.1 −11.45 ± 0.08 0.6 0.14
Ca2+/CaM 2 D # 1.7 × 10−10 ± 2.0 × 10−11 −10.6 ± 0.4 7.4 ± 1.6 −12.68 ± 0.07 200 −3.00

ΔΔ G = RTln (KdIQAAA / KdIQ), where T = 288K

“#” indicates that N was set to 1.

“S” denotes a simple and “D” a displacement titration

‘n’ indicates the number of independent experiments.

“n/a” means not applicable.