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(A) A modular cassette was cloned into pUC18 to generate pSS, the modular cassette contained chloramphenicol resistance gene (cat), UPRTase encoding gene (upp) and the recognition site of I-SceI endonuclease at 3′ end of upp gene. (B) The xylose-inducible I-SceI cassette was cloned into pEB, with a temperature-sensitive replicon (repF) and erythromycin resistance gene (erm).
