Table 1. Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant description(s)a | Reference or source |
| Strains | ||
| E. coli DH5α | Standard cloning strain | Invitrogen |
| B. subtilis 168 | Wild-type strain, trpC2 | Laboratory stock |
| B. subtilis BUK | B. subtilis 168 derivate, Δupp::Para-comK | This study |
| B. subtilis BUK-1C | B. subtilis 168 derivate, Δupp::Para-comK, ΔccpN::upp-cassette | This study |
| B. subtilis BUK-1 | B. subtilis 168 derivate, Δupp::Para-comK, ccpN (Ala 130 to Ser) | This study |
| B. subtilis BUK-2C | B. subtilis 168 derivate, Δupp::Para-comK, ΔccpN::upp-cassette | This study |
| B. subtilis BUK-2 | B. subtilis 168 derivate, Δupp::Para-comK, ΔccpN | This study |
| B. subtilis BUK-3C | B. subtilis 168 derivate, Δupp::Para-comK, ΔileS::upp-cassette-ileS* | This study |
| B. subtilis BUK-3 | B. subtilis 168 derivate, Δupp::Para-comK, ileS (Ala 467 to Thr) | This study |
| B. subtilis BUKΔ20.5 kb | B. subtilis 168 derivate, ΔydcL-yddN | This study |
| B. subtilis BUKΔ75.9 kb | B. subtilis 168 derivate, ΔmutL-pksR | This study |
| Plasmidsb | ||
| pAX01 | B. subtilis integration vector, PxylA, AmpR, EmR | BGSCa |
| pDK | B. subtilis integration vector, KmR | BGSCa |
| pUC18 | E. coli cloning vector, oriT, AmpR | Laboratory stock |
| pTKRED | E. coli cloning vector, I-SceI, SpcR | Laboratory stock |
| pE194 | Bacillus/Staphylococcus cloning vector, EmR | Laboratory stock |
| pSS | Modular vector carrying upp-cassette, AmpR, CmR | This study |
| pST | B. subtilis integration vector, Para-comK, AmpR | This study |
| pEB | B. subtilis-E. coli shuttle vector, AmpR, EmR | This study |
| pEBS | B. subtilis-E. coli shuttle vector, PxylA-I-SceI, AmpR, EmR | This study |
| pEBS-cop1 | pEBS carrying a point mutation in cop-1 site, EmR | This study |
| pSS-ileS | B. subtilis integration vector, CmR | This study |
a
Bacillus Genetic Stock Center.
b
Antibiotic resistance genes in plasmids were abbreviated as follows: AmpR, ampicillin resistance; CmR, chloramphenicol resistance; EmR, erythromycin resistance; SpcR, spectinomycin resistance; KmR, kanamycin resistance.