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. 2013 Nov 25;8(11):e81579. doi: 10.1371/journal.pone.0081579

Figure 6. Effects of insulin and IGF-I stimulation on the mRNA expression levels of epithelial and mesenchymal markers.

Figure 6

(A) The bar graphs show the relative amount of E-cadherin, occludin, β-catenin, vimentin, fibronectin and N-cadherin mRNA levels by qRT-PCR. Significant down-regulation of the mRNA levels of epithelial markers (occludin and β-catenin) and an up-regulation of the mesenchymal marker fibronectin were observed after insulin and IGF-I stimulation. Values were normalized to the amount of mRNA in MDA-MB-435+E-cad. Error bars indicate the means + S.E.M. (n = 3). * = P < 0.05, ** = P < 0.01, ANOVA test. Effects of stimulation with insulin and IGF-I on the fibronectin protein levels. (B) Total cell lysates from MDA-MB-435+E-cad,MDA-MB-435+E-cad stimulated (24h) with insulin or IGF-1, and MDA-MB-435+mock were obtained and analyzed by Western blot for fibronectin. Increased fibronectin expression levels are observed after stimulation with insulin or IGF-I. The bar graphs show the relative amount of fibronectin levels normalized to tubulin.