Figure 3. Analysis of parent-of-origin-specific methylation of the retrocopy in the RB1 gene.

(A) All rhesus macaque family members are heterozygous (G/T), thus, the parental origin of the alleles cannot be resolved. (B) One allele is methylated and the other allele is unmethylated in both individuals studied. (C) The offspring of the marmoset family carries an informative SNP (C/G). (D) The degree of methylation of the supposed maternal allele is 72%, whereas the supposed paternal allele shows 39% methylation. A, C Sanger sequencing of genomic DNA; B, D deep bisulfite sequencing.