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. 2013 Nov 25;8(11):e81502. doi: 10.1371/journal.pone.0081502

Figure 4. Allele-specific methylation and independent monoallelic expression of the retrocopy on chromosome 4 in marmoset.

Figure 4

(A) A SNP (C/G) was used to distinguish the alleles. The mean methylation in the 15 heterozygous individuals was 30-69%, the G allele showed 5-15% methylation and the C allele showed 53-83% methylation. The 5 individuals homozygous for the C allele showed a mean methylation level of 34-66%. The individual homozygous for the G allele (100707) showed no methylation. Thus, the methylation pattern of the retrocopy on marmoset chromosome 4 is allele-specific. red, methylated; blue, unmethylated. (B) By RT-PCR and sequencing, a transcript specific for this retrocopy was identified in 6 heterozygous individuals (no RNA was available from the other heterozygous individuals). This transcript is monoallelically expressed and its expression is not regulated by DNA methylation as transcripts from methylated or unmethylated alleles were obtained.