Figure 5. Expression of the alternative RB1 transcript in rhesus macaque.

By exon-connection RT-PCR of CpG 39 and exon 3 of the rhesus macaque RB1 gene an alternative RB1 transcript was detected. Top left: scheme of rhesus macaque RB1 and position of the primers. Top right: PCR products of RT-PCR and RePCR (reamplification of the RT-PCR product); as there was only a faint band visible after RT-PCR (white circle) RePCR was performed. Sequence analysis of the RT-PCR and RePCR product showed that it is specific for the 2B-transcript (bottom). M, MassRuler low range – Thermo Scientific , +, mRNA with reverse transcriptase (RT); -, mRNA without RT; H₂O, negative control.