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. 2013 Nov 25;8(11):e75162. doi: 10.1371/journal.pone.0075162

Figure 5. Two-dimensional gel electrophoretic separation of extracts from 100 fourth instar larvae and 100 pupae of An. gambiae.

Figure 5

The gel was stained with colloidal Coomassie Brilliant Blue and all the spots migrating with apparent molecular weight lower than 24(coverage by aminoacid sequence up to 61.87%), found in several spots (red circles). In larvae we could also detect OBP21 (Entry code in Uniprot Q8I8S3; coverage by aminoacid sequence 9.16%) and SAP3 (coverage by aminoacid sequence up to 18.25%), present in spots where also OBP9 was identified. Molecular weight markers are, from the top: Phosphorylase b, from rabbit muscle (97 kDa), Bovine serum albumin (66 kDa), Ovalbumin (45 kDa), Carbonic anhydrase (29 kDa), Trypsin inhibitor (20 kDa), α-Lactalbumin (14 kDa).