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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Virology. 2013 Oct 3;447(0):10.1016/j.virol.2013.09.007. doi: 10.1016/j.virol.2013.09.007

Fig. 2.

Fig. 2

Intracellular localization of transcription factors in RK-G8-A1-A2Flag cells. Uninfected RK-G8-A1-A2Flag cells (upper panel) and RK-G8-A1-A2 cells infected with VACV for 7 h (lower panels) were fixed and permeabilized. The samples were stained with a rabbit polyclonal primary antibody to the picornavirus 2A peptide, which remains attached to the G8 and A1 proteins, a mouse anti-Flag MAb to detect FLAG attached to the A2 ORF and the mouse MAb to A14, followed by donkey anti rabbit IgG and goat anti-mouse IgG coupled to Alexa Fluor 488 and Alexa Fluor 594, respectively. The nuclei and cytoplasmic viral factories (arrows) were stained with DAPI.

HHS Vulnerability Disclosure