Table 1.

Dual late activities of intermediate promoters

VACV WR	VACV COP	Relativea luminescence	Functional categoriesb
33	K2L	22,623	Host interaction; EV and WV membrane proteins
35	K4L	21,103	DNA replication, core-associated enzymes and transcriptional factors
52	F13L	556,924	EV and WV membrane proteins
62	E6R	10,679	Required for morphogenesis
63	E7R	0	Unknown
64	E8R	826	Core-associated non-enzymatic proteins
67	E11L	74,499	Core-associated non-enzymatic proteins
69	O2L	249,648	Core-associated enzymes and transcriptional factors
69.5	O3L	55,201	MV membrane-associated proteins; EFC
70	I1L	54,568	Core-associated non-enzymatic proteins; required for morphogenesis; DNA-binding and packing proteins
74	I5L	151,740	MV membrane-associated proteins
75	I6L	33,651	Core-associated non-enzymatic proteins; required for morphogenesis; DNA-binding or packaging proteins
77	I8R	25,296	Core-associated enzymes and transcriptional factors; transcription
81	G4L	210,652	MV membrane-associated proteins; S-S bond formation pathway; required for morphogenesis
86	G8R	20,235	Transcription, late stage
91	L4R	174,828	Core-associated non-enzymatic proteins; DNA-binding proteins; transcription
93	J1R	61,879	Core-associated non-enzymatic proteins
99	H1L	79,812	Core-associated enzymes and transcriptional factors; transcription
101	H3L	120,648	MV membrane-associated proteins
105	H7R	19,760	Contributes to the formation of crescent membrane
111	D6R	74,841	Core-associated enzymes and transcriptional factors; DNA-binding and packing proteins; transcription
113	D8L	388	MV membrane-associated proteins
115	D10R	9,701	Host interaction
116	D11L	45,394	Core-associated enzymes and transcriptional factors; Transcription
118	D13L	160,625	Required for morphogenesis; required for crescent formation
119	A1L	4,072	Transcription, late stage
120	A2L	5,502	Transcription, late stage
122	A3L	372,152	Core-associated non-enzymatic proteins; required for morphogenesis
125	A6L	54,180	Core-associated non-enzymatic proteins; required for morphogenesis
131	A12L	343,579	Core-associated non-enzymatic proteins
135	A15L	113,953	Core-associated non-enzymatic proteins; required for morphogenesis
136	A16L	8,392	MV membrane-associated proteins; EFC
139	A19L	593,009	Unknown
142	A22R	67,366	DNA replication; DNA-binding or packing proteins
146	no ortholog	932	Pseudogene
147	no ortholog	0	Pseudogene
148	A25L	721,492	MV membrane-associated proteins; truncated
150	A27L	198,933	MV membrane-associated proteins
153	A30L	212,505	Core-associated non-enzymatic proteins; required for morphogenesis
153.5	A30.5L	0	Unknown
155	A32L	62,632	Core-associated enzymes and transcriptional factors; DNA-binding or packaging proteins
157	A34R	676,586	EV and WV membrane proteins
162	A38L	17,456	CD47-like putative membrane protein
164	no ortholog	0	Pseudogene
167	A42R	577,110	Unknown
168	A43R	26	Host interaction
179	A53R	506	Host interaction
186	B4R	21,721	Unknown
191	B9R	10,233	Unknown
192	B10R	0	Unknown
197	WR-B15R	134	Host interaction
206	C13L	2,878	Unknown
204.5	no ortholog	112,865	Unknown

^aRelative to activity (1,000,000 fluorescent units) of F17 late promoter

^bEFC, Entry-Fusion Complex; EV, enveloped virion; WV, wrapped virion