Table 1.
Substrates tested for GD NEIL3 activity and binding.
| Lesion | Base excision or binding | Lesion | Base excision or binding |
|---|---|---|---|
| ssAP | +++ | AP:C | + |
| ssGh | +++ | Gh:C | ++ |
| ssSp | +++ | Sp:C | ++ |
| ss5OHC | + | 5OHC:G | − |
| ss5OHU | + | 5OHU:A | − |
| ssDHT | − | DHT:A | − |
| ssDHU | ± | DHU:A | − |
| ssU | − | U:A | − |
| hairpin | − | 8oxoG:C | − |
| 3′flap | − | 5′flap | − |
| A:A mismatch | − | A:TT loop | − |
| 3-Way junction | − | I:T | − |
| εC:G | − | εA:T | − |
| 1 ssrA | − | 1 rA:T | − |
| 1 ssrU | − | 1 rU:A | − |
| 1 ssrC | − | 1 rC:G | − |
| 1 ssrG | − | 1 rG:C | − |
| ssHmc | − | HmC:G | − |
| Ss5meC | − | 5meC:G | − |
Abbreviations: 3′flap, DNA duplex with single stranded 3′ overhang; 3-way junction, replication intermediate, 5′flap, DNA duplex with single stranded 5′overhang; 5meC, 5-methylcytosine; 5OHC, 5-hydroxycytosine; 5OHU, 5-hydroxyuracil; A:TT, insertion loop; εA, 1,N6-ethenoadenine; εC, 3,NA-ethenocytosine; DHT, 5,6-dihydrothymine; DHU, 5,6-dihydroxyuracil; Gh, guanidinohydantoin; HmC, 5-hydroxymethylcytosine; I, inosine; Sp, spiroiminodihydantoin;
1
DNA oligo with one ribonucleotide incorporated.