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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: J Immunol Methods. 2013 Sep 18;0:10.1016/j.jim.2013.09.006. doi: 10.1016/j.jim.2013.09.006

Figure 6.

Figure 6

Bivalent porcine IL-2 fusion toxin depletes porcine CD25+ LCL13271 cells in vitro. Porcine CD25+ B-cell lymphoma cells (LCL13271) were incubated with the indicated concentrations of the Bi-NonGly fusion toxin (DT390-bi-pIL-2-Non-N-Gly). Reduction in CD25+ cells following treatment with the fusion toxin was measured by flow cytometry using anti-porcine CD25 mAb (Clone 231-3B2) at 24 h (left panel), 48 h (middle panel) and 72 h (right panel). Cyclohexamide (1:8) was used as positive control. Data are representative of multiple individual experiments.