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Analysis of the porcine IL-2 fusion toxins’ ability to bind to CD25+ porcine PBMC in vitro. Porcine PBMC were stained with biotinylated porcine IL-2 fusion toxins and analyzed by flow cytometry. Biotinylated anti-porcine CD25 mAb (clone 231-3B2) was included as positive control and biotinylated porcine CD3 εγ (Peraino et al., 2012b) was utilized as a control for background due to biotinylation. Data are representative of multiple individual assays.
