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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Genesis. 2013 Aug 30;51(11):10.1002/dvg.22420. doi: 10.1002/dvg.22420

Figure 2. CFP expression is restricted to the blood islands in E8.5 ε-globin-H2B-CFP embryos.

Figure 2

(a) Frontal (upper panels) and transverse (lower panels) cryosections of E8.5 ε-globin-H2B-CFP embryos showing CFP fluorescence in the blood islands. Images were acquired using a Zeiss AxioCam monochrome camera mounted on a Zeiss Axio Observer Z1 inverted microscope outfitted with a Plan-Neofluar 5×/0.15 or an EC Plan-Neofluar 10×/0.30 objective. (b) Images of a PECAM1-PE stained E8.5 ε-globin-H2B-CFP embryo (upper panels) and yolk sac (lower panels) showing CFP fluorescence exclusively within the developing vasculature. Images of whole embryos were acquired using a Zeiss AxioCam monochrome camera mounted on a Zeiss Lumar V12 stereomicroscope equipped with a NeoLumar S 1.5X FWD 30 mm objective. Photographs of yolk sac details were taken using a Zeiss Axio Observer Z1 inverted microscope outfitted with a Plan-Apochromat 20×/0.8 objective. Scale bars, 100 μm.