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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Genesis. 2013 Aug 30;51(11):10.1002/dvg.22420. doi: 10.1002/dvg.22420

Figure 5. Multicolor analysis of H2B-CFP in combination with GFP.

Figure 5

(a) FACS analysis of peripheral blood from E14.5 ε-globin-H2B-GFP and ε-globin-H2B-CFP embryos and of a mixed blood sample showing good separation of the fluorescent signals from the two transgenes. (b) Images of a double transgenic embryo (ε-globin-H2B-CFP; Afp-GFP) showing clear separation of CFP and GFP. Photographs were taken using a Zeiss AxioCam monochrome camera mounted on a Zeiss Axio Observer Z1 inverted microscope outfitted with a Plan-Neofluar 5x/0.15 objective. Scale bar, 200 μm.