Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Genesis. 2013 Aug 30;51(11):10.1002/dvg.22420. doi: 10.1002/dvg.22420

Figure 8. E14.5 placental macrophages engulf primitive erythroid cells nuclei.

Figure 8

Fluorescence images of F4/80 stained fetal liver (a) and placenta (c) sections from E14.5 ε-globin-H2B-CFP embryos, with magnified views of the boxed regions. DAPI staining of the sections is also shown. Flow cytometry plots of F4/80 stained cells from dispersed fetal liver (b) and placenta (d) of E14.5 ε-globin-H2B-CFP embryos. (c) Images of F4/80 positive macrophages with engulfed CFP-positive nuclei. Arrowheads indicate the spaces occupied by the CFP-positive nuclei inside the macrophages. Photographs were taken using a Zeiss AxioCam monochrome camera mounted on a Zeiss Axio Observer Z1 inverted microscope outfitted with a Plan-Apochromat 20x/0.8 objective (a and c) or a Plan-Apochromat 63x/1.4 oil objective (e). Scale bars, 20 μm.