Table 1.

Effect of inhibitors on inhibition of the maturation of SUMO precursors by SENP1 and SENP2

Compounds			IC50(μM) - SUM01		IC50(μM) - SUM02
Structure	Code†	NCI ID‡	SENP1	SENP2	SENP1	SENP2
	SPI-01	NSC5068	32.8 ± 1.82	1.42 ± 3.0	1.88 ±2.2	1.1 ± 5.8
	SPI-02	NSC16224	26.5 ± 1.86	3.42 ± 1.6	2.08 ± 2.0	2.70 ± 2.1
	SPI-03	NSC8676	20.27 ± 2.47	5.17 ± 1.32	1.86 ± 2.3	3.0 ± 2.0
	SPI-04	NSC34933	11.2 ± 1.7	1.6 ± 2.5	2.32 ±2.6	2.15 ± 2.28
	SPI-05	NSC5067	> 60	19.7 ± 1.47	7.5 ± 1.6	4.6 ± 1.65
	SPI-06	NSC70551	> 60	3.62 ± 1.98	43.2 ± 2.2	10.7 ± 1.6
	SPI-07	NSC58046	> 60	> 60	17.54 ± 4.9	28.06 ± 9.2
	SPI-08	NSC22940	> 60	4.1 ± 3.0	> 60	41.06 ± 5.2
	SPI-09	NSC42164	> 60	23.36 ± 1.6	> 60	26.96 ± 2.5
	SPI-10	NSC45551	> 60	34.21 ± 1.9	11.1 ± 3.7	36.44 ± 5.7

^†Designation for our library of SUMO-protease inhibitors (SPI)

^‡Designated by the National Cancer Institute