. Author manuscript; available in PMC: 2014 Oct 1.

Published in final edited form as: Radiother Oncol. 2013 Sep 7;109(1):10.1016/j.radonc.2013.08.015. doi: 10.1016/j.radonc.2013.08.015

Table 1.

Morphometric measurements of A549 lung tumor nodules from mice treated with soy and radiation

	Nodule Area^a mean±SE×10⁴ μm²	Range^b (×10⁴ μm²)	Tumor Area^c (%)	Ki-67+ Nuclei^d mean±SE
Control	70 ± 8	31–180	62 ± 4	147 ± 17
Soy	18 ± 3	4–52	32 ± 7	50 ± 8
Radiation	10 ± 4	1–35	17 ± 5	38 ± 6
Rad + Soy	3 ± 1	1–8	8 ± 2	16 ± 3

The surface areas of lung tumor nodules were estimated by morphometric measurements of each nodule in an average of 20 fields, using H&E stained lung tissue sections obtained from 3 mice per treatment group. In control and soy-treated groups, we are reporting the mean of 100 representative measurable tumor nodules and the mean of 24 tumor nodules in radiation-treated group. In radiation + soy -treated groups (Rad + Soy), 19 fields were analyzed showing 0–2 tumors per field. The mean tumor nodule area ± SE is reported for each treatment group.

The ranges of tumor nodule areas are presented.

The percent tumor area was calculated by dividing the area covered with tumor nodules by the total area of the field comprising tumor nodules and lung tissue. The area covered with tumor nodules was obtained by the sum of all nodules measured in each field. The area of lung tissue surrounding the tumor nodules was morphometrically measured in all analyzed fields. The mean percent tumor area ± SE is reported.

The number of Ki-67 positive nuclei in tumor cells was enumerated in representative tumor nodules. The mean positive nuclei per tumor nodule ± SE is reported.