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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Radiother Oncol. 2013 Sep 7;109(1):10.1016/j.radonc.2013.08.015. doi: 10.1016/j.radonc.2013.08.015

Table 2.

Quantitation of alveolar septa thickness in lungs from mice treated with soy and radiation

Ratio of Alveolar Septa Area/20X Field
Treatment Regular Septaa Thick Septab Regular Septac
Area Ratio Range 0.3 – 0.50 0.51 – 0.65 0.3–0.50
Proportion Percent Proportion Percent graphic file with name nihms523974t1.jpg
Tumor-Bearing Lungs Control 13/20 65% 7/20 35%
Radiation 11/20 55% 9/20 45%
Soy 17/20 85% 3/20 15% Thick Septad
0.51–0.65
Radiation + Soy 14/20 70% 6/20 30% graphic file with name nihms523974t2.jpg
Normal Lung 16/20 80% 4/20 20%

Using software analysis of H&E slides, the ratio of alveolar septa area relative to the total area of 20X field was quantified in 20 fields of 20X while contouring and excluding bronchioles and large vessels (see insetc). Data were stratified by using a ratio of 0.3–0.50 to define regular normal septaa, c and 0.51–0.65 to define thick septab, d which is indicative of pneumonitis (see insetd). Data computed on lungs from 2 representative mice are presented.