Fig. 2.

A schematic illustration of scaffold biofunctionalisation techniques

Electrospun PLLA scaffolds are plasma treated and then functionalised with LTGF by two methods:random and orientated approaches. PLLA, poly(L-lactide); TGF, transforming growth factor; sulfo-SMCC, sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate; LTGF, latent transforming growth factor-β1; pLTGF, random immobilisation of latent TGF-β1 on plasma treated PLLA scaffold; sLTGF, orientated immobilisation of latent TGF-β1 using sulfo-SMCC on plasma treated PLLA scaffold.