Fig. 6.

Cell viability of human chondrocyte culture

The cell viability of primary human chondrocytes cultured on various scaffold types at 1, 7, and 14 days is shown (scale bar=200 µm). The numbers shown in each image indicate the mean percentage±standard deviation. The average number of cells per image on day 1, 7 and 14 were approximately 30, 50, and 30, respectively. PLLA, untreated poly(L-lactide) scaffold; ptPLLA, plasma treated PLLA scaffold; TGF, ptPLLA with supplementation of 10 ng/mL active TGF-β1 for up to 7 days; pLTGF, random immobilisation of latent TGF-β1 on plasma treated PLLA scaffold; sLTGF, orientated immobilisation of latent TGF-β1 using sulfo-SMCC on plasma treated PLLA scaffold; sulfo-SMCC, sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate.