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. 2013 Sep 11;305(10):C1069–C1079. doi: 10.1152/ajpcell.00080.2013

Fig. 3.

Fig. 3.

Effect of NOX5-S on acid-induced DNMT1 expression. A: in BAR-T cells, acid treatment significantly increased DNMT1 mRNA expression. Knockdown of NOX5-S with NOX5-S siRNA blocked acid-induced increase in DNMT1 mRNA expression. B: in CP-A cells, acid treatment significantly increased DNMT1 mRNA expression. Knockdown of NOX5-S with NOX5-S siRNA blocked acid-induced increase in DNMT1 mRNA expression. C: in FLO cells, acid treatment significantly increased DNMT1 mRNA expression. Knockdown of NOX5-S with NOX5-S siRNA blocked acid-induced increase in DNMT1 mRNA expression. D: a reporter plasmid of DNMT1 (pGL3-DNMT1P) was generated by ligating a DNMT1 promoter fragment (−1751 to 0 from ATG) into the pGL3-basic vector. Acid treatment significantly increased luciferase activity, an increase that was significantly reduced by knockdown of NOX5-S. E: overexpression of NOX5-S significantly increased luciferase activity in BAR-T cells. These data suggest that acid-induced increase in DNMT1 expression and promoter activity may depend on activation of NOX5-S (n = 3). ANOVA: *P < 0.001, compared with control siRNA group; ▲P < 0.05, ▲▲P < 0.001, compared with control siRNA + acid group. **P < 0.05 by t-test.