Fig. 4.

Acid-induced activation of NF-κB in BAR-T cells. A typical example of Western blot analysis (A) and summarized data (B) showed that acid treatment significantly decreased the expression of IκBα, when compared with the control group. Data suggest that acid treatment may activate NF-κB in BAR-T cells. C: acid treatment significantly increased the luciferase activity in BAR-T cells transfected with NF-κB cis-reporter plasmid pNF-κB-Luc, which contains 5 repeats of NF-κB binding element GGGGACTTTCC in the enhancer element of the plasmid. NOX5 siRNA significantly decreased acid-induced increase in luciferase activity in BAR-T cells. These data suggest that acid-induced activation of NF-κB may depend on activation of NOX5-S in BAR-T cells (n = 3). ANOVA: *P = 0.001, compared with control siRNA group; **P < 0.05, compared with control siRNA + acid group. #P < 0.001 by t-test.