Fig. 7.

Restoration of thermal hyperalgesia in PRL KO female mice with hindpaw administration of mouse PR and lack of structural changes in PRL-R KO mice. A: CFA-induced heat hyperalgesia was partially restored in PRL KO but not WT female mice by administration of 10 μg mouse PRL in 6-h post-CFA time point into hindpaws (ΔPWL; **P < 0.01; 1-way ANOVA; n = 5). B: CFA-induced cold hyperalgesia was partially restored in PRL KO but not WT female mice by administration of 10 μg mouse PRL in CFA-injected hindpaws (ΔTemperature°C; *P < 0.05 and **P < 0.01; 1-way ANOVA; n = 5). Model, posttreatment with vehicle or PRL, and mouse lines are indicated below x-axis in A and B. Heat and cold nociception was measured 30 min after vehicle (saline) administration, and 30 or 60 min after PRL administration. C: no obvious structural changes are present in the dorsal horn of the lumbar spinal cord of PRL-R KO female and male mice compared with WT male or female mice. Transverse sections of the spinal cord sections were stained with hematoxylin and eosin.