Fig. 1.

Chub-S7 cell line validation. A: quantitative real-time PCR mRNA expression of the brown adipose tissue (BAT) marker uncoupling protein 1 (UCP1) in Chub-S7 preadipocytes (Chub Pre; n = 3) and differentiated Chub-S7 adipocytes (Chub Adip; n = 3), mature human subcutaneous adipocytes (Sc Adip; n = 8), human primary differentiated preadipocytes without (Sc Pre Ctr; n = 3) or with dehydroepiandrosterone (DHEA) (Sc Pre DHEA; 100 nM and 10 μM, each n = 3), human primary hepatocytes (Hep; n = 4), mouse BAT (mBAT; n = 3), mouse white adipose tissue (mWAT; n = 4), and hepatocytes (mHep; n = 3). B: mRNA expression of the white adipose tissue marker fatty acid-binding protein 4 (FABP4) in Chub-S7 and human tissues as before. Chub-S7 cells, similarly to other non-BAT tissues, did not express any substantial amounts of UCP1 but showed FABP4 expression similar to white adipose tissue. Data expressed as mean arbitrary units (AU) ± SE from triplicate measurements. STS, steroid sulfatase.